Wednesday, April 20, 2011

pGLO Transformation

Thems is spooky cats! For the past 2 days we've been working on a pGLO Transformation Lab. It's basically playing with E. coli and seeing which one glows on the 2nd day, like the cats above. Don't worry they didn't put E. coli in those cats, but it is a similar process. But they are both genetically modified.

Here's an example of the glowing sample from a lab done in another class.

So what made the bacteria glow? Well, we began the lab with 2 samples, a positive and negative solution. After about 20 minutes of work, we were dishing out samples into 4 different agar sample plates (LB+, LB/Amp/ara+, LB/Amp+, and LB/Amp-). We waited over night and in the morning checked for results. Using a little black light we ran it over the 4 samples. As you can tell from the wonderful picture to the left, LB/Amp/ara was the completely awesome glowing sample. We successfully modified the bacteria! Hurrah for playing with bacteria!

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